Sometimes good comedy is like good music, you love it so much that you put it on repeat. You insist on bugging your friends to watch or listen, since you take so much delight in it surely they will as well! It's the bit that you quote or reference ad nauseam. What is that bit for you?
Hannibal Burress's bit about ordering a second line tickles me every time I hear it. The sheer ridiculousness and how he tells the story makes me kind of wish he would move to New Orleans for comedic effect. I could listen to it a dozen times over.
And while Tig Notaro's most famous bit now is her multiple run ins with Taylor Dane, nothing beats the simplicity of how hilarious she is just moving a stool.